Colloid biology

Colloid: a mixture of microscopically dispersed insoluble PARTICLES suspended throughout another substance. The PARTICLES, usually inanimate, have different physical and chemical properties, which determine their characteristics.

BIOcolloid = a mixture of CELLS ! I.e. live particles that sense the environment, respond to it and change with time. Meaning, they are a bit more complex to study and understand. All hands on deck!

NEWS FROM THE LAB

WHAT WE ARE WORKING ON

Using polyelectrolytes to modify cell surface charge and induce cell attachment.

Multi-species artificial aggregates and biofilms that mimic natural structures. 

Carriers with and active role in promotion of bacterial physiology.

Building complex multi-step biotransformation pathways involving multiple partners.

Characterizing bacterial strains on multiple levels using OMICS. 

Detection of toxic metals like mercury by whole-cells or specific proteins.

APPLIED KNOWLEDGE FOR

Bacterial cell factories with complex metabolic pathways, aggregated cellular structures in 2D (biofilms) or 3D (spherical aggregates)

Bioremediation, biotransformation

Guiding bacterial cells in vivo, bacterial production of medical compounds

Decomposition of waste, bacterial fertilizers

Protective coatings for materials, or coatings that interact and modify the material


THE TOOLS WE USE

PARTICLE SIZE & CHARGE CHARACTERIZATION

CELL ENTRAPMENT & ENCAPSULATION

ELECTROSTATIC MODIFICATION OF SURFACES

FLOW CYTOMETRY

CUSTOM MADE MICROFLUIDICS

QCM

TIME LAPSE FLUORESCENCE MICROSCOPY

SCANNING ELECTRON MICROSCOPY & EDS

RAMAN SPECTROSCOPY

GAS & LIQUID CHROM. MASS SPECTROSCOPY

FLOUR. & LUMINESCENCE SPECTROSCOPY

MICROSENSORS

MOLECULAR CLONING

REAL-TIME QUANTITATIVE PCR

NANOPORE SEQUENCING

BIOINFOMRATICS (OMICS)

MACHINE LEARNING

METABOLIC MODELING

THE TEAM

Aleš Lapanje

Associate professor
Head of the Lab for Colloid Biology

PhD in Microbiology

Establishing the new scientific field - Colloid Biology

Tomaž Rijavec

Assistant Professor

PhD. in Molecular Biology

Working on bacterial communities, genes & genomes 

Anatolii Abalymov

Postdoctoral Researcher

PhD in Biotechnologies

Working on development of materials for tissue engineering, lab-on-chip systems, nano- and microparticles for drug delivery

Tayebeh Sharifi 

Postdoctoral Researcher

PhD in Material Sciences

Working on design and development of nanomaterials for a hybrid of bio-photo-electrochemical system for detritiation of light-water and simultaneously H2 generation.

Maja Zugan

PhD candidate

Msc in Microbiology

Working on environmental applications of colloid biology

Dmitrii Deev

PhD candidate

M.Sc. in Biology

Working on dynamics of artificial biofilms

Farzaneh Hosseini

PhD candidate

Msc in Chemistry

Working on microbe driven enrichment of tritium

Allwin Mabes Raj

PhD candidate

Msc in Zoology (spl. Biotechnology)

Working on Nanobiosensors for methylmercury detection 

Tamara Kerševan

Msc. Candidate

Studying the relations of particle size and microbial biofilms.

Urša Lovše

Msc. Biochemistry

Working on genome sequencing and characterization.

Teja Colja

Msc. candidate

Working on characteristation of antimicrobial activity of bacterial isolates

OUR CLOSE COLLABORATORS @ JSI

Marko Štrok

PhD Chemistry

Laboratory for radiochemistry

Tina Kosjek

PhD Chemistry

Laboratory for analytical chemistry - Organic Analysis

Jitka Hreščak

PhD Biology

Center for Electron Microscopy and Microanalysis

Raghuraj S Chouhan

PhD Chemistry

Laboratory for nanomateials

Milena Horvat

PhD Chemistry

Department of Environmental Sciences Head

Tomaž Lutman

PhD Chemistry

Technology Manger

SELECTED PAPERS:

Grilc, N.K., Zidar, A., Kocbek, P., Rijavec, T., Colja, T., Lapanje, A., Jeras, M., Gobec, M., Mlinarič-Raščan, I., Gašperlin, M. and Kristl, J., 2023. Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity. Journal of Controlled Release, 355, pp.371-384.

Verdel, N., Sežun, M., Rijavec, T., Zugan, M., Deev, D., Rybkin, I. and Lapanje, A., 2023. Microbiotechnology-Based Solutions for Removal and Valorization of Waste in Pulp and Paper Industry. In Biorefinery for Water and Wastewater Treatment (pp. 445-479). Cham: Springer International Publishing.

González-Plaza, J.J., Furlan, C., Rijavec, T., Lapanje, A., Barros, R., Tamayo-Ramos, J.A. and Suarez-Diez, M., 2022. Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels. Frontiers in Microbiology, 13.

Deev, D., Rybkin, I., Rijavec, T., Lapanje, A. 2021. When Beneficial Biofilm on Material Is Needed: Electrostatic Attachment of Living Bacterial Cells Induces Biofilm Formation. Frontiers in Materials. doi:10.3389/fmats.2021.624631 

van Tatenhove-Pel, R.J., Rijavec, T., Lapanje, A. et al. Microbial competition reduces metabolic interaction distances to the low µm-range. ISME J 15, 688–701 (2021). https://doi.org/10.1038/s41396-020-00806-9

Rybkin, I., Gorin, D., Sukhorukov, G., Lapanje, A. Thickness of Polyelectrolyte Layers of Separately Confined Bacteria Alters Key Physiological Parameters on a Single Cell Level. Frontiers in Bioengineering and Biotechnology, doi:10.3389/fbioe.2019.00378

Rijavec, T., Zrimec, J., van Spanning, R., Lapanje, A., Natural Microbial Communities Can Be Manipulated by Artificially Constructed Biofilms. Adv. Sci. 2019, 6, 1901408. https://doi.org/10.1002/advs.201901408

LATEST UPDATES

ONGOING PROJECTS

SurfBio (Horizon 2020)

Innovation hub for surface and colloid biology research - SEE MORE -

BioSysmo (Horizon 2020)

BIOremediation systems exploiting SYnergieS for improved removal of Mixed pOllutants - SEE MORE

Integrated systems for effective environmental remediation - SEE MORE

Crossing (HZDR, Germany)

Crossing Borders and Scales - an interdisciplinary approach

Valorisation of olive oil waste material by microbial multispecies biocatalytic aggregates

New biofertilization approach based on the microbial multispecies biocatalytic aggregates

ARRS (J7-2597) Biotrisep 

Cost-efficient separation of tritium from water with bio-based systems  

Innovative isotopic techniques for identification of sources and biogeochemical cycling of mercury in contaminated sites  

Phoenix (COST ACT. CA19123)

Protection, Resilience, Rehabilitation of damaged environment

Cycling of substances in the environment, mass balances, modelling of environmental processes and risk assessment  

INTERNATIONAL NETWORK

UNIVERSITY OF BURGOS

GHENT UNIVERSITY

WAGENINGEN 

UNIVERSITY & RESEARCH

HELMHOLZ ZENTRUM 

DRESDEN-ROSENDORF

VU AMSTERDAM

DELFT UNIVERSITY OF TECHNOLOGY

LEITAT

IDENER

INSTITUTE OF AGRICULTURE AND TOURISM

QUEEN MARY UNIVERSITY

TOMSK UNIVERSITY

SARATOV STATE UNIVERSITY

UNIVERSITY OF BELGRADE

 VRIJE UNIVERSITEIT BRUSSEL

NATIONAL NETWORK

CHEMICAL INSTITUTE

UNIVERSITY OF LJUBLJANA

UNIVERSITY OF MARIBOR

UNIVERSITY OF PRIMORSKA

UNIVERSITY OF NOVO MESTO

LOCATION

GROUP FOR COLLOID BIOLOGY
DEPARTMENT OF ENVIRONMENTAL SCIENCES
JOŽEF STEFAN INSTITUTE | REACTOR CENTER PODGORICA
BRINJE 40, 1262 DOL PRI LJUBLJANI, SLOVENIA